Sunday, August 14, 2016
First Week of Class 8/8/16 - 8/12/16
This week, we worked on making and sterilizing KAN. Kanamycin (KAN) is an antibiotic that kills bacteria but the bacteria we have been working with (E.coli ) is KAN- resistant. Our TA's have been teaching us about all the machines in the lab and how to use them. Most of our work is done under the flow hood and that just keeps our bacteria or whatever we are working on from being contaminated. Earlier in the week we worked on our plate streaking techniques. You can't just streak all over the plates, if you do then you won't be able to get an isolated colony like you really want and need to experiment with. Last year, when I was a freshman, in Biology we did this experiment where we streaked bacteria onto a plate, each plate was different so we picked one plate, then we had to choose a temperature. The outcome was the bacteria was supposed to glow in the dark if put in the right conditions. Our TA's gave us a preview of the pGLO Transformation, Now we have to do the same thing but just on our own in groups of 4 without anybody telling us exactly what to do.